Emerging resistance of van genotype in enterococci: A potential menace for therapeutic failure

Emerging van genotype in enterococci

Keywords: Vancomycin resistant enterococci, E. faecalis, E. faecium, Glycopeptide resistance, van genotypes, Linezolid


Objectives: Emerging cases of vancomycin-resistant enterococci (VRE) are detrimental for the patients. The current study aimed to ascertain the occurrence of VRE, their antibiogram and the van genotype responsible for vancomycin resistance.

Methods: A total number of 2,958 clinical specimens were processed at Microbiology Department of the Alrazi Health Care, Lahore during the one year (2016-2017) using microbiological culture media, biochemical and serology. Antibiogram of enterococcal strains was performed using disc diffusion and E-test. ATCC Enterococcus faecalis 29212 was used as a quality control strain. The detection of van genotypes was accomplished by multiplex PCR assay.

Results: Out of the 147 enterococci, 139 (94.6%) were E. faecalis, and 8 (5.4%) were E. faecium. Statistically significant associations of urine (p < 0.001), pus (p < 0.001) and wound swabs (p = 0.001) were observed with E. faecalis. A significant correlation of enterococcal infections (p = 0.05) was seen with female patients. Four (2.9%) strains of E. faecalis found to be VRE with vanB (75%) and vanA (25%) genotypes.

Conclusion: The emerging strains of VRE (vanB and vanA genotype) in the current study are a potential menace for therapeutic failure, which left the physicians with only linezolid as a therapeutic option.

doi: https://doi.org/10.12669/pjms.35.6.1145

How to cite this:
Ejaz H. Emerging resistance of van genotype in enterococci: A potential menace for therapeutic failure. Pak J Med Sci. 2019;35(6):1659-1663. doi: https://doi.org/10.12669/pjms.35.6.1145

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Author Biography

Hasan Ejaz, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University

Assistant Professor of Microbiology

Department of Clinical Laboratory Sciences

College of Applied Medical Sciences

Jouf University, Sakaka, Saudi Arabia


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