Ameloplasty is counterproductive in reducing microleakage around Resin Modified Glass Ionomer and Resin based fissure sealants

Is ameloplasty beneficial for sealants retention?

  • Tabinda Nawaz Khan Dow University of Health Sciences
  • Farhan Raza Khan, Dr Aga Khan University & Hospital
  • Syed Yawar Ali Abidi, Dr Jinnah Sind Medical University,
Keywords: Ameloplasty, Microleakage, Sealants, RMGIC, Flowable composite resin


Objective: To compare the microleakage around resin modified glass ionomer cement (RMGIC) based sealants and flowable resin based sealants placed with or without ameloplasty in extracted human teeth.

Methods: This in-vitro experimental study was conducted at the Operative Dentistry Department, Dow University of Health Sciences, Karachi, Pakistan from June 2017 to December 2018. Sixty extracted human molars and premolars were assigned to four groups (n=15) each, according to the type of fissure sealant (flowable resin based sealant or resin modified glass ionomer based sealant) used and either placed with or without ameloplasty. Specimens were thermocycled and then immersed in 1% methylene blue for 24 hours. Specimens were then sectioned and examined using stereo-microscope (50X) for microleakage that was scored on an ordinal scale. Mann-Whitney U test and Ordinal regression were applied. Level of significance kept at 0.05.

Results: There was a statistically significant difference (p-value <0.001) between the two sealant types for the microleakage scores. Sealants placed with ameloplasty demonstrated significantly higher microleakage values (p-value <0.001).

Conclusion: Microleakage was found to be more pronounced in RMGIC based sealants compared to the resin based sealants. Ameloplasty resulted in higher leakage around the sealants irrespective of the chemistry of material.


How to cite this:
Khan TN, Khan FR, Abidi SYA. Ameloplasty is counterproductive in reducing microleakage around Resin Modified Glass Ionomer and Resin based fissure sealants. Pak J Med Sci. 2020;36(3):544-549. doi:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Author Biographies

Farhan Raza Khan, Dr, Aga Khan University & Hospital


Associate Professor, Operative Dentistry

Dental Section, Department of Surgery,

Aga Khan University, Karachi Pakistan,

Syed Yawar Ali Abidi, Dr, Jinnah Sind Medical University,


Professor & Head, Dept. of Operative Dentistry

Sind Institute of Oral Health Sciences

Jinnah Sind Medical University, Karachi, Pakistan

Original Articles