Expression of p-AMPK in colorectal cancer revealed substantial diverse survival patterns

Expression of p-AMPK in colorectal cancer

  • Mohamad Nidal Khabaz King Abdulaziz University
  • Amer Shafei Abdelrahman
  • Jaudah Ahmad Al-Maghrabi King Abdulaziz University
Keywords: Phosphorylated AMPK, p-AMPK, Immunohistochemistry, Colorectal cancer


Objective: Several cancers have showed differences in the role of p- AMPK in cancer growth, progression and prognosis, and little is identified regarding the significance of p-AMPK expression in colorectal adenocarcinoma. Therefore, this report will define p-AMPK phenotype in a panel of colorectal carcinomas and explore the relationship between this phenotype and tumor clinicopathological features.

Methods: A total of 228 cases comprising 155 large intestine cancers and 73 controls (40 benign tumors and thirty three non-cancerous tissues) were employed in tissue microarray construction. Immunohistochemistry (IHC) staining was applied to reveal p-AMPK expression. This study was carried out in the pathology lab of King Abdulaziz University Hospital over a duration of 15 months and was completed on 7th July 2018.

Results: Phosphorylated AMPK was identified in 133 (85.8%) of colorectal cancers and 73 (100%) control cases. Histologic type was noticeably correlated with p-AMPK immunostaining (P= 0.001), high score of p-AMPK immunostaining is more frequent in control cases. Considerable varied survival models were observed with neoplasm size, metastatic tumor, recurrence and disease relapse (P-values <0.01). Survival estimates are considerably healthier in positive cases which have one of the following features size less than 5 cm, absence of metastatic tumor, no reoccurrence or disease relapse.

Conclusions: The present study showed a reduction in the IHC staining of p-AMPK in colorectal cancer compared with controls. IHC staining of p-AMPK can be a supportive marker in predicting prognosis and survival estimates of colorectal tumors with specific clinical factors.


How to cite this:
Khabaz MN, Abdelrahman AS, Al-Maghrabi JA. Expression of p-AMPK in colorectal cancer revealed substantial diverse survival patterns. Pak J Med Sci. 2019;35(3):685-690. doi:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Author Biography

Jaudah Ahmad Al-Maghrabi, King Abdulaziz University

Department of Pathology, Faculty of Medicine

King Abdulaziz University

Jeddah, Saudi Arabia.

How to Cite
Khabaz, M. N., Abdelrahman, A., & Al-Maghrabi, J. (2019). Expression of p-AMPK in colorectal cancer revealed substantial diverse survival patterns. Pakistan Journal of Medical Sciences, 35(3).
Original Articles