Immunohistochemical Expression of MMP-9 and E-Cadherin in subtypes of Ameloblastoma

  • Farah Farhan, Dr Foundation University College of Dentistry, Islamabad
  • Zainab Niazi, Dr Islamabad Medical and Dental College, Islamabad
  • Sana Masood, Dr Foundation University College of Dentistry, Islamabad
  • Beenish Abbas, Dr Foundation University College of Dentistry, Islamabad
Keywords: Ameloblastoma, E-Cadherin, Matrix metalloproteinases, Histopathology

Abstract

Background & Objective: Ameloblastomas have been analyzed histologically for diagnostics and its sub-classification; however, the analysis carried out so far does not provide any authentic evidence regarding prognosis of Ameloblastoma. Subject categorization is still a topic of debate. The purpose of this study was to determine the immuno-expression of markers such as MMP-9 and E-Cadherin in different sub-types of ameloblastoma and establish their correlation with histological variants.

Methods: Analytical study of 71 cases of ameloblastoma was conducted in AFIP Rawalpindi, between January to June, 2019. Samples were taken from different intraoral sites including the patients with tumor of ameloblast. The tumor was sub classified histologically on the basis of WHO classification. ‘Chi Square’ Test was applied to find the association of MMP-9 and E-Cadherin with histological variants of ameloblastoma. P-value ≤ 0.05 were found statistically significant.

Results: On histopathological sub-classification, 52.1% were diagnosed as follicular type, 23.9% were plexiform type, 14.1% were Acanthomatous type and 9.9% were of unicystic ameloblastoma. 80% of the total Acanthomatous type and 59% of the total plexiform had strong immuno-expression, which was significantly different from follicular type MMP-9 (p ≤ 0.05). All cases of unicystic ameloblastoma and 67.6% of follicular type showed strong immuno-expression significantly different from 20% of Acanthomatous type and 59% of plexiform type E-Cadherin (p ≥ 0.05). On the other hand, when statistical analysis was carried out, an inverse relation between MMP-9 and E-cadherin was observed.

Conclusion: The effect of MMP-9 and E-cadherin in ameloblastoma is aggressive in nature and effectiveness was seen in subtypes of ameloblastoma.

doi: https://doi.org/10.12669/pjms.38.1.4465

How to cite this:
Farhan F, Niazi Z, Masood S, Abbas B. Immunohistochemical Expression of MMP-9 and E-Cadherin in subtypes of Ameloblastoma. Pak J Med Sci. 2022;38(1):207-213. doi: https://doi.org/10.12669/pjms.38.1.4465

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Author Biographies

Farah Farhan, Dr, Foundation University College of Dentistry, Islamabad

M.Phil (Oral pathology)

Assistant professor

Zainab Niazi, Dr, Islamabad Medical and Dental College, Islamabad

M.Phil (Oral pathology)

Assistant professor

Sana Masood, Dr, Foundation University College of Dentistry, Islamabad

MSc, DPH, BDS (Community dentistry)

Assistant professor

Beenish Abbas, Dr, Foundation University College of Dentistry, Islamabad

FCPS (Operative dentistry)

Assistant professor

Published
2021-11-22
How to Cite
Farhan, F., Niazi, Z., Masood, S., & Abbas, B. (2021). Immunohistochemical Expression of MMP-9 and E-Cadherin in subtypes of Ameloblastoma. Pakistan Journal of Medical Sciences, 38(1). https://doi.org/10.12669/pjms.38.1.4465
Section
Original Articles