Prognostic markers in Chronic Lymphocytic Leukaemia - A flow cytometric analysis

Prognostic markers in Chronic Lymphocytic Leukaemia

  • Hala Haq Doctor
  • Nasir Uddin
  • Saleem Ahmed Khan
  • Sunia Ghaffar
Keywords: Chronic Lymphocytic Leukaemia, Prognostic markers, Immunophenotyping, CD39, ZAP-70, CD49d

Abstract

Objective: To find out the frequency of ZAP-70, CD38 and CD49d in patients diagnosed with CLL in our population.

Methods: This is a cross sectional study conducted in Army Medical College in collaboration with Armed Forces Institute of Pathology and Military Hospital Rawalpindi from 1st January 2018 to 30th November 2018. Permission from Institutional Ethical Committee was obtained. Blood samples were collected by non-probability consecutive sampling technique and analyzed for blood counts and flow cytometry was done for ZAP-70, CD38 and CD49d. Manufacturer’s instructions for the kits were strictly followed.

Results: Fifty-one newly diagnosed patients with CLL were studied for the prognostic markers in CLL. CD 38 was expressed in 25(49%) and CD49d in 21(41.2%). ZAP-70 expression was not detected in our series of patients.

Conclusion: We conclude that CD38 and CD49d expression was detected in almost half of the patients of CLL in our series. CD49d showed statistically positive correlation with CD38, showing that it is a more pragmatic choice for reliable prognostication of CLL along with CD38.

doi: https://doi.org/10.12669/pjms.36.3.541

How to cite this:
Haq H, Uddin N, Khan SA, Sunia Ghaffar4. Prognostic markers in Chronic Lymphocytic Leukaemia - A flow cytometric analysis. Pak J Med Sci. 2020;36(3):338-343. doi: https://doi.org/10.12669/pjms.36.3.541

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published
2020-03-03
Section
Original Articles