Detection of extended-spectrum ?-lactamases in Klebsiella pneumoniae: Comparison of phenotypic characterization methods

Authors

  • Hasan Ejaz GC University, Lahore, Pakistan.
  • Ikram ul Haq GC University, Lahore.
  • Saqib Mahmood University of Health Sciences, Lahore.
  • Aizza Zafar The Children’s Hospital & Institute of Child Health, Lahore.
  • Muhammad Mohsin Javed GC University, Lahore,

DOI:

https://doi.org/10.12669/pjms.293.3576

Keywords:

ESBL, Detection methods, Phenotypic characterization of ESBL, DDST and CLSI detection methods.

Abstract

Objective: Extended-spectrum ?-lactamase producing K. pneumoniae is a serious threat to the patients. This manuscript shows the comparison of phenotypic characterization methods used for ESBL K. pneumoniae and frequency distribution of these isolates in various clinical samples.

Methodology: Eleven different types of pathological samples collected on various time intervals were analyzed. K. pneumoniae were identified with API 20E system (bioMerieux) and initial screening of ESBL K. pneumoniae was performed using the ceftazidime antimicrobial disc. Double-disc synergy test (DDST) and CLSI confirmatory test were compared for the phenotypic detection of ESBL K. pneumoniae.

Results: A total number of 214 ESBL producing K. pneumoniae were isolated from various clinical samples. Frequency distribution of ESBL producing K. pneumoniae was found to be highest among blood 117 (54.7%) and urine 46 (21.5%) samples. Data regarding the use of various interventions among these patients showed most common presence of intravenous line 209 (97.7%) and urinary catheters 46 (21.5%). Comparison of DDST and CLSI confirmatory test showed that the DDST detected 145 (67.8%) isolates while 213 (99.5%) ESBL K. pneumoniae were characterized by CLSI confirmatory test.

Conclusion: The use of CLSI confirmatory test is very efficient in the early detection of ESBL K. pneumoniae especially when the facilities for molecular characterization are not available.

doi: http://dx.doi.org/10.12669/pjms.293.357

6How to cite this:Ejaz H, Ikram-ul-Haq, Mahmood S,Zafar A, Javed MM. Detection of extended-spectrum ?-lactamases in Klebsiella pneumoniae: Comparison of phenotypic characterization methods. Pak J Med Sci 2013;29(3):768-772.

Author Biographies

Hasan Ejaz, GC University, Lahore, Pakistan.

Institute of Industrial Biotechnology,

Ph Research Scholar

Ikram ul Haq, GC University, Lahore.

Institute of Industrial Biotechnology,

Director

Saqib Mahmood, University of Health Sciences, Lahore.

Department of Human Genetics and Molecular Biology,

Assistant Professor

Aizza Zafar, The Children’s Hospital & Institute of Child Health, Lahore.

Department of Microbiology,

Associate Professor

Muhammad Mohsin Javed, GC University, Lahore,

Institute of Industrial Biotechnology,

Associate Professor

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Published

2013-05-21

How to Cite

Ejaz, H., Haq, I. ul, Mahmood, S., Zafar, A., & Javed, M. M. (2013). Detection of extended-spectrum ?-lactamases in Klebsiella pneumoniae: Comparison of phenotypic characterization methods. Pakistan Journal of Medical Sciences, 29(3). https://doi.org/10.12669/pjms.293.3576

Issue

Vol. 29 No. 3 (2013): June 2013 Pak J Med Sci

Section

Original Articles