Rapid screening of pyogenic Staphylococcus aureus for confirmation of genus and species, methicillin resistance and virulence factors by using two novel multiplex PCR

Authors

  • Abdul Haque Postgraduate Research Laboratory, The University of Faisalabad, Faisalabad, Pakistan
  • Asma Haque Department of Bioinformatics and Biotechnology, GC University, Faisalabad, Faisalabad, Pakistan
  • Muhammad Saeed Postgraduate Research Laboratory, The University of Faisalabad, Faisalabad, Pakistan
  • Aysha Azhar Postgraduate Research Laboratory, The University of Faisalabad, Faisalabad, Pakistan
  • Samreen Rasool Postgraduate Research Laboratory, The University of Faisalabad, Faisalabad, Pakistan
  • Sidra Shan Postgraduate Research Laboratory, The University of Faisalabad, Faisalabad, Pakistan
  • Beenish Ehsan Department of Bioinformatics and Biotechnology, GC University, Faisalabad, Faisalabad, Pakistan
  • Zohaib Nisar Department of Bioinformatics and Biotechnology, GC University, Faisalabad, Faisalabad, Pakistan

DOI:

https://doi.org/10.12669/pjms.335.13487

Keywords:

Pyogenic staph, MRSA, Virulence factors, Multiplex PCR.

Abstract

Objectives: Emergence of methicillin resistant Staphylococcus aureus (MRSA) is a major medical problem of current era. These bacteria are resistant to most drugs and rapid diagnosis can provide a clear guideline to clinicians. They possess specific virulence factors and relevant information can be very useful. We designed this study to develop multiplex PCRs to provide rapid information.

Methods: We studied 60 Staphylococcus aureus isolates and detected methicillin resistance by cefoxitin sensitivity and targeting of mecA gene. After initial studies with uniplex PCRs we optimized two multiplex PCRs with highly reproducible results. The first multiplex PCR was developed to confirm genus, species and methicillin resistance simultaneously, and the second multiplex PCR was for screening of virulence factors.

Results: We found 38.33% isolates as methicillin resistant. ?-toxin, the major cytotoxic factor, was detected in 40% whereas ?-hemolysin was found in 25% cases. Panton Valentine leucocidin was detected in 8.33% and toxic shock syndrome toxin in5% cases. The results of uniplex and multiplex PCRs were highly compatible.

Conclusions: These two multiplex PCRs when run simultaneously can provide vital information about methicillin resistance and virulence status of the isolate within a few hours as compared to several days needed by routine procedures.

doi: https://doi.org/10.12669/pjms.335.13487

How to cite this:Haque A, Haque A, Saeed M, Azhar A, Rasool S, Shan S, et al. Rapid screening of pyogenic Staphylococcus aureus for confirmation of genus and species, methicillin resistance and virulence factors by using two novel multiplex PCR. Pak J Med Sci. 2017;33(5):1095-1100.

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Published

2017-10-19

How to Cite

Haque, A., Haque, A., Saeed, M., Azhar, A., Rasool, S., Shan, S., … Nisar, Z. (2017). Rapid screening of pyogenic Staphylococcus aureus for confirmation of genus and species, methicillin resistance and virulence factors by using two novel multiplex PCR. Pakistan Journal of Medical Sciences, 33(5). https://doi.org/10.12669/pjms.335.13487

Issue

Vol. 33 No. 5 (2017): September – October 2017

Section

Original Articles