Genetic polymorphism of the prostasin gene in hypertensive pregnant Pakistani females

  • Saima Ejaz King Saud BIn Abdul Aziz University of Health Sciences
  • Anwar Ali Department of Physiology, University of Karachi, Pakistan
  • Sumaira Riffat Sindh Medical College, Jinnah Sindh Medical University, Karachi, Pakistan
  • Atif Mahmood Department of Physiology, Bhitai Medical and Dental College, Mirpur Khas, Pakistan
  • Kamran Azim Department of Life Sciences, Muhammed Ali Jinnah University
Keywords: Hypertension, Prostasin, Epithelial Sodium Channel

Abstract

Objective: The study was performed to investigate the association of hypertension in pregnancy with prostasin gene polymorphism in Pakistani females.

Methods: This case-control study was performed at University of Karachi, Pakistan from April 2018 to May 2019. A total of 160 females, including 90 hypertensives and 70 healthy pregnant females, were recruited by purposive sampling after obtaining informed written consent. Genotyping was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).

Results: The frequencies of the TC and CC genotypes were higher in hypertensive pregnant females compared to healthy controls. A significant difference was evident for CC (P=0.012) genotype; however, no significant difference was observed for TC (P=0.49) and TT genotypes (P=0.06) between control and hypertensive groups. The adjusted odds ratio for CC genotype was 6.2 (P=0.025) and 1.48 (P=0.44) for TC genotype compared to the TT genotype. There was a significantly higher prevalence of the C allele of the prostasin gene at rs12597511 in the hypertensive group, suggesting that this allele is a risk factor for hypertension and cardiovascular diseases.

Conclusion: C allele at rs12597511 of prostasin gene demonstrate as a risk factor for having hypertension in pregnancy.

doi: https://doi.org/10.12669/pjms.37.1.3666

How to cite this:
Ejaz S, Ali A, Riffat S, Mahmood A, Azim K. Genetic polymorphism of the prostasin gene in hypertensive pregnant Pakistani females. Pak J Med Sci. 2021;37(1):109-113. doi: https://doi.org/10.12669/pjms.37.1.3666

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published
2020-12-08
How to Cite
Ejaz, S., Ali, A., Riffat, S., Mahmood, A., & Azim, K. (2020). Genetic polymorphism of the prostasin gene in hypertensive pregnant Pakistani females. Pakistan Journal of Medical Sciences, 37(1). https://doi.org/10.12669/pjms.37.1.3666