Acute and subacute toxicity studies of a poly herbal formulation used for diabetes

  • Aymen Owais Ghauri Jinnah University for Women
  • Ejaz Mohiuddin Hamdard University
  • Tayyeba Rehman The Islamia University Bahawalpur
  • Sheraz Muhammad Siddiqui Hamdard University
Keywords: PHF-dia, Poly herbal formulation, Diabetes, Toxicity


Objectives: PHF-dia (Poly Herbal Formulation Diabetes) is a polyhedral formulation possessing antihyperglycemic and antihyperlipdimic effects. This study aims to assess acute and sub-acute toxicity of PHF-dia in rats.

Methods: This is an experimental study conducted in two different phases. Acute toxicity was conducted for 14 days and sub-acute toxicity was conducted for 28 days. Both studies were conducted in animal house of Jinnah University for Women, Karachi, Pakistan. Acute toxicity was evaluated in vivo with single time oral administration of 400 mg/kg and 2000 mg/kg doses for two weeks. Sub-acute toxicity was investigated with the application of repeated doses of 150 mg/kg/day, 250 mg/kg/day and 500 mg/kg/day for 28 days.

Results: Acute toxicity study results showed no toxic symptoms, behavioral changes or death in rats up to 2000 mg/kg. Therefore, LD50 of oral toxic dose must be more than 2000 mg/ml. Similarly, sub-acute toxicity studies confirmed the safety of PHF-dia and showed no clinical symptoms nor biochemical or histological variation in rats treated with 150 mg/kg, 250 mg/kg and 500 mg/kg compared to the control group (p <0.05).

Conclusion: This indicates safe nature of PHF-dia for the further clinical trials. However, mechanism of action of PHF-dia is not fully understood.

ANOVA: Analysis of variance, ALT: Alanine transaminase, AST: Aspartate transaminase,
EDTA: Ethylene diamine tetra-acetic acid, H & E: Haematoxylin and Eosin,
HDL: High density lipoprotein, LD50: Median lethal dose, LDL: Low density lipoprotein,
PLT: Platelet count, RBC: Red blood cell, SPSS: Statistical package for social science,
VLDL: Very low-density lipoprotein, WBC: White blood cell, PHF-dia (Poly Herbal Formulation Diabetes).


How to cite this:
Ghauri AO, Mohiuddin E, Rehman T, Siddiqui HSM. Acute and subacute toxicity studies of a poly herbal formulation used for diabetes. Pak J Med Sci. 2022;38(6):1668-1673. doi:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Author Biographies

Ejaz Mohiuddin, Hamdard University

Prof. Dr. Ejaz Mohiuddin, Chairman, Department of Surgery & Allied Sciences (JARAHAT VA MULHIQA ULUM), Faculty of Eastern Medicine, Hamdard University

Tayyeba Rehman, The Islamia University Bahawalpur

Tayyeba Rehman

Associate lecturer,

University College of Conventional Medicine, Faculty of Medicine, and Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur (63100), Pakistan

Sheraz Muhammad Siddiqui, Hamdard University

Sheraz Muhammad Siddiqui, Chairman, Department of Community Medicine & Behavioural Sciences (SAMAJI SEHAT-VA-ILMUL-KHASAIL), Faculty of Eastern Medicine, Hamdard University

How to Cite
Ghauri, A. O., Mohiuddin, E., Tayyeba Rehman, & Sheraz Muhammad Siddiqui. (2022). Acute and subacute toxicity studies of a poly herbal formulation used for diabetes. Pakistan Journal of Medical Sciences, 38(6).
Original Articles