Zinc-alpha 2 glycoprotein a diagnostic Biomarker for early stage oral Squamous Cell Carcinoma

  • Dr. Mehwish Feroz Ali Ziauddin University
  • Dr. Mervyn Hosein Ziauddin University
  • Dr. Saima Butt Ziauddin University
  • Dr. Rehan Siddiqui Ziauddin University
Keywords: Zinc alpha-2 glycoprotein, Mouth neoplasm, Adipokines, Neoplasm staging, Immunohistochemistry


Objectives: In this study, we investigated the expression of zinc alpha-2 glycoprotein in oral squamous cell carcinoma tissue samples. Additionally, ascertained its association to the oral cancer stage and subscale parameters (TNM).

Methods: This observational study was conducted at Ziauddin University from January to December 2020. Using the Open-Epi software, the sample size of 120 oral squamous cell carcinomas was calculated at 95% confidence interval and a 5% margin of error. Ethical approval was taken from the Institutional Ethical Review Committee. Histologically diagnosed cases of oral squamous cell carcinoma were obtained from the Histopathology Department of Ziauddin University, Karachi. Study data was analyzed through SPSS version-20 and p-value ≤0.05 considered as significant. One-way ANOVA and Multiple linear regression were applied for analysis of data.

Results: In the study, none of the oral squamous cell carcinoma tissue samples from the later stages were stained for ZAG. However 71% (35/49) of the early stage OSCC samples showed positive IHC results for ZAG expression in the cytoplasm. One-way ANOVA indicates that high ZAG expression was significantly associated with smaller tumor size (p<0.001), lymph node involvement (p=0.002), early stages of OSCC (p<0.001) and less differentiated tumor (p=0.001). The site of the tumor was also significantly associated with ZAG staining (p<0.001).

Conclusion: Zinc alpha-2 glycoprotein expressed in the early stages of oral cancer development so that effective treatment modalities can be planned as per the patient’s status. This may also assist a clinician to achieve tumor-free surgical margins and monitor the post treatment outcomes.

doi: https://doi.org/10.12669/pjms.39.2.6488

How to cite this: Ali MF, Hosein M, Butt S, Siddiqui R. Zinc-alpha 2 glycoprotein a diagnostic Biomarker for early stage oral Squamous Cell Carcinoma. Pak J Med Sci. 2023;39(2):513-517. doi: https://doi.org/10.12669/pjms.39.2.6488

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to Cite
Feroz Ali, D. M., Hosein, D. M., Butt, D. S., & Siddiqui, D. R. (2023). Zinc-alpha 2 glycoprotein a diagnostic Biomarker for early stage oral Squamous Cell Carcinoma. Pakistan Journal of Medical Sciences, 39(2). https://doi.org/10.12669/pjms.39.2.6488
Original Articles